Impact of seeding time and Pratylenchus neglectus on Rhizoctonia fungicide yield responses (SARDI215)


July 1, 2015


June 30, 2016


Factors that may affect crop response to fungicide for Rhizoctonia root rot were studied in the SA Mallee in 2015. Time of sowing was the greatest influencer of crop yield, however it did not affect the response of Rhizoctonia to fungicide application. Fungicide application is more likely to have a positive effect on yield in barley compared to wheat. In wheat, moderate levels of Pratylenchus neglectus reduced the effectiveness of fungicide application, though it had no effect in barley.


Rhizoctonia root rot, caused by Rhizoctonia solani AG8, is a significant soil-borne disease in cereals, particularly in lower rainfall areas and sandy soils. Previous SAGIT/GRDC funded research has shown that new fungicides can provide useful control of rhizoctonia root rot, and that yield responses increased when growing season rainfall increased from 200 to 400mm rainfall.

This is the first project designed to study the effects of time of sowing and Pratylenchus neglectus on the yield responses to Uniform® applied to control rhizoctonia root rot. The project was carried out at Wilkawatt, in the South Australian Mallee during the 2015 growing season.

Research Aims

The core objectives of the project were to:
• Determine if time of sowing and/or moderate numbers of the root lesion nematode Pratylenchus neglectus affected the yield response of wheat and barley to the application of Uniform® fungicide.
• Provide recommendations to growers on the likely impact of factors on yield responses associated with the application of fungicides to control rhizoctonia root rot.

In The Field

The growing season at Wilkawatt in 2015 had 114mm rainfall, a dry spring (26mm rainfall) and cold soil temperatures which dropped to 10oC four times in May. The cold start probably contributed to the development of Rhizoctonia patches.

Scope barley and Grenade CL Plus wheat were sown into two P. neglectus densities averaging two and 10 nematodes per gram soil. There were three times of sowing – 6 May, 22 May and 11 June. Note that the ‘higher’ nematode treatment represents a moderately low nematode density in the southern region.

Uniform fungicide was applied as a dual treatment on the furrow surface (200ml/ha) and in furrow (200ml/ha) to the ‘+fungicide’ plots. All plots received Di-Ammonium Phosphate (DAP) at 50kg/ha coated with Flutriafol; Boxer Gold® was applied at seeding to control ryegrass. The site was harvested in late November and yields of each plot measured.

Plots were soil sampled on the previous crop row prior to sowing, and on and between rows in August and October, and on rows post-harvest in November. Rhizoctonia and P. neglectus levels were measured at each sampling time using the SARDI PREDICTA® B DNA soil testing service.


While the time of sowing influenced crop yields, it did not impact the effectiveness of fungicide treatment in either wheat or barley. Wheat and barley sown 6 May produced 2.01 tonnes per hectare (t/ha) and 2.4t/ha respectively, dropping to 1.40t/a and 1.20t/ha by 11 June.

The fungicide improved yield in barley by 0.3t/ha, with no significant interaction with time of sowing.

Where P. neglectus levels were 2/g soil, fungicide increased wheat yield by 0.2t/h, but no yield increase was seen where P. neglectus levels were 10/g soil. A similar effect was observed at Lameroo in 2014 in the separate SAGIT project Field trials to assess the efficacy of farm practices associated with Fungicide control of Rhizoctonia. The fungicide treatment had no overall effect on grain quality.

Soil testing found that fungicide treatment reduced Rhizoctonia levels by 30 to 40 per cent on row, and increased root growth by more than 50 per cent in wheat and 40 per cent in barley. Fungicide did not affect Rhizoctonia levels or root growth between rows.

NDVI data captured by drones in August showed significant correlation between biomass and yield and a negative correlation between uneven growth and yield. This indicates that yield was determined in August, and minimising uneven growth could reduce yield losses. The fungicide application reduced uneven growth in barley but not wheat.

These results need to be confirmed at other sites and in different seasonal conditions.

Project Participants

SARDI: Dr Alan McKay, Dr Katherine Linsell and Dr Victor Sadras
University of Adelaide: Dr Ramesh Raja Segaran
Rho Environmetrics: Dr Ray Corell

The Problem

The factors that cause yield responses to vary when fungicides are applied to control rhizoctonia root rot have not been well understood.

The research

The project studied the effect of time of sowing and levels of the root lesion nematode Pratylenchus neglectus on wheat and barley crop responses to Uniform® fungicide in a paddock with high Rhizoctonia levels at Wilkawatt in 2015.

More information

Dr Alan McKay, SARDI
T: 08 8303 9375
E: [email protected]

Value for Growers

The key findings for growers are:
• Time of sowing did not influence fungicide effectiveness but still had the greatest effect on yields in wheat and barley.
• Fungicide application to control rhizoctonia root rot is more likely to produce a yield response in barley than wheat.
• The fungicide treatment increased barley yield by 0.3t/ha even in a low rainfall year.
• Moderately low levels of root lesion nematode P. neglectus can reduce the yield response from fungicide application in wheat in a low rainfall growing season.
• Reducing uneven growth can reduce yield losses from Rhizoctonia.